اثر متیل‌ جاسمونات و استرادیول بر شاخص‌های فیزیولوژیکی و خاصیت آنتی‌اکسیدانی گل داروئی همیشه‌بهار (Calendula officinalis L.)

نوع مقاله : مقاله تحقیقاتی

نویسندگان

گروه باغبانی، دانشگاه آزاد اسلامی واحد رشت، رشت، گیلان، ایران

چکیده

همیشه بهار (Calendula officinalis L.) از خانواده Asteraceae و گیاهی زینتی- داروئی زیبا با منشاء مدیترانه و غرب آسیا و اروپای مرکزی می‌باشد. به منظور بررسی اثر متیل جاسمونات و استرادیول بر شاخص­های رویشی و خاصیت آنتی­اکسیدانی گل همیشه بهار، آزمایشی فاکتوریل بر پایه بلوک‌های کامل تصادفی در بهار 1395، با دو فاکتور متیل جاسمونات در 4 غلظت (0، 10، 100 و 500 میکرومولار) و استرادیول در 4 سطح (0، 1، 5 و 10 میلی‌لیتر در گرم) در 3 تکرار در گلخانه دانشگاه آزاد اسلامی واحد رشت انجام شد. نتایج نشان داد که اثر ساده متیل جاسمونات در غلظت‌های 10 و 500 میکرومولار به­ ترتیب بر صفات تعداد برگ، کلروفیل کل، کاروتنوئید گلبرگ و ظرفیت آنتی‌اکسیدانی بیشترین اثر را داشته است. همچنین، اثر ساده استرادیول در غلظت‌های 10 و 5 میلی‌لیتر برگرم به ترتیب بر صفات تعداد برگ، وزن خشک، آنزیم پراکسیداز، فلاونویید طول موج‌های 300 و330 نانومتر و آنتوسیانین، دارای بیشترین اثر بوده است. همچنین نتایج این آزمایش نشان داد که بیشترین تعداد برگ، بیشترین میزان کلروفیل a و b و آنزیم پراکسیداز تحت تیمار متیل‌جاسمونات 500 میکرومولار و استرادیول 10 میلی‌لیتر در ‌گرم به­دست آمد.

چکیده تصویری

اثر متیل‌ جاسمونات و استرادیول بر شاخص‌های فیزیولوژیکی و خاصیت آنتی‌اکسیدانی گل داروئی همیشه‌بهار (Calendula officinalis L.)

تازه های تحقیق

نتایج بدست آمده از این آزمایش نشان داد که متیل‌جاسمونات در غلظت‌های 10 و 500 میکرومولار و استرادیول در غلظت­های 5 و 10 میلی­لیتر بر گرم بیشترین تأثیر را روی صفات اندازه‌گیری شده در این آزمایش داشته است. همچنین نتایج حاصل از اثر متقابل "متیل‌جاسمونات × استرادیول" نشان داد که بیشترین تعداد برگ، بیشترین میزان کلروفیل a، بیشترین میزان کلروفیل b و آنزیم پراکسیداز تحت تیمار متیل‌جاسمونات 500 میکرومولار و استرادیول 10 میلی‌لیتر بر ‌گرم و بیشترین میزان ظرفیت آنتی‌اکسیدانی گیاه تحت تیمار متیل‌جاسمونات 500 میکرومولار و استرادیول 5 میلی‌لیتر بر‌ گرم به دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Physiological and antioxidant properties of marigold (Calendula officinalis L.) as influenced by methyl jasmonate and estradiol

نویسندگان [English]

  • Shahram Sedaghat Hoor
  • Fatemeh Raof Hagh Parvar
Department of Horticulture, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Introduction: Marigold (Calendula officinalis L.) is an ornamental and medicinal plant from the family Asteraceae that is originated from the Mediterranean region, Western Asia, and Central Europe. The application of steroids to plants is an interesting subject. Steroid hormones, such as estradiol, stimulate growth and development and root and stem elongation. Mammalian sex hormones (progesterone, β-estradiol, and androsterone) stimulate antioxidant systems and enhance the growth of plants. Jasmonic acid and methyl jasmonate (MeJA) widely occur in plants and are involved in a wide range of processes, including fruit ripening, the production of viable and active pollens, root growth, tendril coiling, and protection against pathogens and insects. The present research aimed to investigate the effects of methyl jasmonate (MeJA) and estradiol on some physiological traits, including vegetative parameters and antioxidant properties of marigold.
Experimental: The study carried out as a factorial experiment based on a Randomized Complete Block Design with two factors including MeJA at four rates (0, 10, 100 and 500 μM) and estradiol at four rates (0, 1, 5 and 10 mg/l) with 16 treatments, 3 replications, and 48 plots. The growth regulators were used as the foliar application at three phases in 2-week intervals. The recorded traits included leaf number, plant fresh and dry weight, anthocyanin, carotenoid, chlorophyll a, b and total, catalase activity, peroxidase enzyme, petal flavonoid, and antioxidant property.
Results: The analysis of variance revealed that the simple effect of MeJA was significant on leaf number, antioxidant capacity, total chlorophyll, and petal carotenoid. Also, the simple effect of estradiol was significant on leaf number, plant dry weight, anthocyanin, peroxidase enzyme, and flavonoid. It was found that MeJA × estradiol was significant for leaf number, total chlorophyll, petal carotenoid, anthocyanin, catalase enzyme activity, peroxidase enzyme activity, antioxidant capacity, plant dry weight, and chlorophyll a and b content. The results showed that MeJA at the rate of 10 μM was most effective in the number of leaves and total chlorophyll. At the rate of 500 μM, it was also effective in the antioxidant capacity. Estradiol at the rate of 10 mg/l was the best treatment for dry weight, peroxidase activity, and flavonoid content. It was observed that 10 μM MeJA × 10 mg/l estradiol resulted in the highest leaf number and chlorophyll content and 500 μM MeJA × 10 mg/l estradiol was related to the highest chlorophyll b content and peroxidase enzyme activity. According to the results, it is recommended to apply estradiol and MeJA to improve the pigments and antioxidant capacity of Calendula officinalis. Dry weight and leaf number were increased with estradiol application. The results confirmed that the application of naturally occurring compounds, like MeJA and steroids, can increase secondary metabolites such as flavonoids and antioxidant enzymes and activity.
Conclusion: To further learn about plant regulators in the future, it is recommended to examine the impact of MeJA and steroids on other plants under biotic and abiotic stresses.
Extension: Based on the results, growers can apply estradiol and MeJA to improve the pigments and antioxidant capacity of Calendula officinalis.

کلیدواژه‌ها [English]

  • Antioxidant
  • Carotenoid
  • estradiol
  • Marigold
  • Jasmonate
Addy, S. K. and Goodman, R. N. 1972. Polyphenol oxidase and peroxidase activity in apple leaves inoculated with a virulent or an avirulent strain of Erwinia amylovora, Indian Phytopathology, 25: 575-579.
Babst, B. A., Ferrieri, R. A., Gray, D. W., Lerdau, M., Schlyer, D.J., Schueller, M., Thrope, M. R. and Orians, C. M. 2005. Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus. New phytologist, 167: 63-72. DOI: https://doi.org/10.1111/j.1469-8137.2005.01388.x
Balbi, V., and Devoto, A. 2008.  Jasmonate signaling network in Arabidopsis thaliana, crucial regulatory modes and new physiological scenarios, New phytologist, 171: 301-318. DOI: https://doi.org/10.1111/j.1469-8137. 2007. 02292.x   
Clouse, S.D. 2019. Brassinosteroids. Reference Module in Biomedical Sciences, https://doi.org/10.1016/B978-0-12-801238-3.11363-7
Creelman, R.A. and Mullet, J.E. 1997. Biosynthesis and action of jasmonates in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 48: 355 – 381. DOI: https://doi.org/10.1146/annurev.arplant. 48.1. 355
Dazy, M., Jung, V., Ferard, J. and Masfaraud, J. 2008. Ecological recovery of vegetation on a coke-factory soil: Role of plant antioxidant enzymes and possible implication in site restoration, Chemosphere, 74: 57-63. DOI: https://doi.org/10.1016/j.chemosphere.2008.09.014
Erdal, S., and Dumlupinar, R. 2011. Mammalian sex hormones stimulate antioxidant system and enhance growth of chickpea plants. Acta Physiologiae Plantarum, 33:1011–1017. DOI: https://doi.org/10.1007/s11738-010-0634-3
Farazi, E., Afshari, H. and Hokm Abadi, H. 2015. Effect of Different Concentrations of Brassinosteroid on Physiomorphological Chracteristics of Five Pistachio Genotypes (Pistacia Vera. L). Nuts, 6(2): 143-153. (In Persian)
Ghosh, P.K., Ajay, Bandyopadhyay, K.K., Manna, M.C., Mandel, K.G., Misra, A.K. and Hati, K.M. 2004. Comparative effectiveness of cattle manure, poultry manure, phosphocompost and fertilizer-NPK on three cropping systems in vertisols of semi-arid tropics. Bioresource Technology, 95: 85-93. DOI: https://doi.org/ 10.1016/j.biortech.2004.02.012
Gong, Y., Liu, X., He, W.H., Xu, H.G., Yuan, F. and Gao, Y.X., 2012. Investigation into the antioxidant activity and chemical composition of alcoholic extracts from defatted marigold (Tagetes erecta L.) residue. Fitoterapia, 83: 481-489. DOI: https://doi.org/10.1016/j.fitote.2011.12.013
Haubrick, L.L. and Assmann, S.M. 2006. Brassinosteroids and plant function: some clues, more puzzles. Plant, Cell and Environment, 29: 446-457. DOI: https://doi.org/10.1111/j.1365-3040.2005.01481.x
Hendrawati, O., Yao, Q., Kim, H.K., Linthorst, H.J.M., Erkelens, C., Lefeber, A.W.M., Choi, Y.H. and Verpoorte, R. 2006. Metabolic differentiation of Arabidopsis treated with methyl jasmonate using nuclear magnetic resonance spectroscopy, Plant Science, 170:1118–1124. DOI: https://doi.org/10.1016/j.plantsci.2006.01.017
Huang, K.L., Miyajima, I., Okubo, H., Shen, T.M. and Huang, T.S. 2001. Flower colours and pigments in hybrid tuberose (Polianthes). Scientia Horticulturae, 88: 235-241. DOI: https://doi.org/10.1016/S0304-4238(00) 00213-2
Janeczko, A. 2000. Influence of selected steroids on plant physiological processes especially flowering induction. PhD Dissertation, Agricultural University, Krakow, Poland.
Janeczko, A., Filek, W., Biesaga-Koscielniak, J., Marcinska, I. and Janeczko, Z. 2003. The influence of animal sex hormones on the induction of flowering in Arabidopsis thaliana: comparison with the effect of 24-epibrassinolide. Plant, Cell Tissue and Organ Culture, 72: 147-151. DOI: https://doi.org/10.1023/A: 1022291718398
Janeczko, A. and Skocczowski, A. 2005. Mammalian sex hormones in plants. Folia Histochemica ET Cytobiologica, 43(2): 71 – 79.
Jason, J.G., Thomas, G.R. and Pharr, D.M. 2004. Photosynthesis, chlorophyll fluorescence, and carbohydrate content of illicum taxa grown under varied irradiance. The American Society for Horticultural Science, 129: 46-53. DOI: https://doi.org/10.21273/JASHS.129.1.0046 
Jia, Z-S., Tang, M.C. and Wu J.M. 1999. The Determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.
Jung, S. 2004.  Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant Physiology and Biochemistry, 42: 231-255. DOI: https://doi.org/10. 1016/j.plaphy.2004.01.001
Krizek, D.T., Kramer, G.F., Upadyaya, A., Mireeki, R.M. 1993. UV-B response of cucumber seedlings grown under metal halide and high pressure sodium/deluxe lamps. Physiologia Plantarum, 88: 350-358. DOI: https://doi. org/10.1111/j.1399-3054.1993.tb05509.x
Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi. J., Sriranganayakulu, G., Reddy, P.C. and, Sudhakar, C. 2006.  Jasmonic acid induced changes in protein pattern, antioxidativeanzyme activities and Peraoxidase isozyme in Peanut seedlings. Biologia Plantarum, 50: 219-226. DOI: https://doi.org/10.1007/s10535-006-0010-8
Mazumdar, B.C. and Majumder, K. 2003. Methods on Physicochemical analysis of fruits. Daya Publishing House. Delhi, India: 137-138.
Milic, B.L., Dilas, S.M. and Ganadanovic- Brunet J.M. 1998. Antioxidative activity of phenolic compounds on the metal-ion breakdown of lipid peroxidation system. Food Chemistry, 61: 443-447. DOI: https://doi.org/10. 1016/s0308-8146(97)00126-x
Mudalige, R.G., Kuehnle, A.R. and Amore, T.D. 2003. Pigment distribution and epidermal cell shape in dendrobium species and hybrids. HortScience, 38: 573–577. DOI: https://doi.org/10.21273/HORTSCI.38.4.573
Norastehnia, A. and Nojavan-Asghari, M. 2006. Effects of methyl jasmonate on the enzymatic antioxidant defense system in maize seedlings subjected to paraquat. Asian Journal of Plant Sciences, 5: 17-23. DOI: https://doi. org/10.3923/ajps.2006.17.23
Omidbaigi, R. Sadrai Menjili, K. and Sefidkon, F. 2006. Effect of Sowing Dates in the Productivity of Fennel (Foeniculum vulgare) CV. Soroksari. Medicinal and Aromatic Plants Research, 21(4): 465-479. DOI: https://doi.org/10.22092/ijmapr.2006.115029. (In Persian)
Ozdamir. F., Bor, M., Demiral, T. and Turkan, I. 2004. Effects of 24 – epibrassinolide on seed germination, seedling growth, lipid Peroxidation, proline content and antioxidative system of rice (Oriza sativa L.) under salinity stress. Plant growth Regulation, 42: 203 – 211. DOI: https://doi.org/10.1023/b:grow.0000026509.25995.13    
Ramandeep, K.T. and Savage, P.G. 2005. Antioxidant activity in different fractions of tomatoes. Food Research International, 38: 487-494.
Raouf Fard, F., Sharifi, M., Omidbaigi, R., Sefidkon, F., Behmanesh, M. and Ahmadi, N. 2014. Effect of methyl jasmonate on metabolic enzymes and phenolics, in Agastache foeniculum [Pursh] Kuntze. Medicinal and Aromatic Plants, 30(3): 361-369. (In Persian)
Reyes, L. and Cisneros-Zevallos, F. 2003. Wounding stress increases the phenolic content and antioxidant capacity of purple flesh potatoes. Agriculture and Food Chemistry, 51: 5296-5300. DOI: https://doi.org/10.1021/ jf034213u
Reyes-Díaz, M., Lobos, T., Cardemil, L., Nunes-Nesi, A., Retamales, J., Jaakola, L., Alberdi, M., Ribera-Fonseca, A. 2016. Methyl jasmonate: an alternative for improving the quality and health properties of fresh fruits. Molecules, 21(6): 567-575. DOI: https://doi.org/10.3390/molecules21060567
Sestak, Z. 1996. Limitations for finding a linear relationship between chlorophyll content and photosynthetic activity. Biologia Plantarum, 8: 336-346. DOI: https://doi.org/10.1007/bf02930670
Shan, X., Zhang, Y., Peng, W., Wang, Z. and Xie, D. 2009. Molecular mechanism for jasmonate- induction of anthocyanin accumulation in Arabidopsis. Experimental Botany, 60: 3849-3860. DOI: https://doi.org/10. 1093/jxb/erp223   
Shore, L.S., Gurevitz, M. and Shemesh, M. 1998. Estrogen as an environmental pollutant. Bulletin of Environmental Contamination and Toxicology, 51: 361-366. DOI:https://doi.org/10.1007/bf00201753
Wittmann, C., Aschan, G. and Pfanz, H. 2001. Leaf and twig photosynthesis if young beech (Fagus sylvatica) and aspen (Populus tremula) trees grown under different light regime. Basic and Applied Ecology, 2: 145-154. DOI: https://doi.org/10.1078/1439-1791-00047.